Peptide Reconstitution / Dosage Calculator

Work out the concentration after reconstitution and how many units to draw on a U-100 insulin syringe for a prescribed dose.

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mg
mL
Enter the dose your prescriber gave you. This tool does not suggest doses.
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How to use this calculator

Enter your values. The result updates as you type; press Calculate to confirm and jump to the result on mobile. Use Share to send the calculator with your result.

Reconstitution math

concentration (mcg/mL) = vial mg × 1,000 ÷ mL of diluent volume (mL) = dose (mcg) ÷ concentration U-100 units = volume (mL) × 100

Example: a 5 mg vial mixed with 2 mL gives 2,500 mcg/mL. A prescribed 250 mcg dose is 250 ÷ 2,500 = 0.1 mL, which is 10 units on a U-100 insulin syringe.

Reading a U-100 syringe

U-100 insulin syringes are marked in units where 100 units = 1 mL, so each unit is 0.01 mL. A 0.3 mL syringe holds 30 units (often with half-unit marks), a 0.5 mL syringe 50 units and a 1 mL syringe 100 units. Pick a syringe where your dose fills a good part of the barrel for easier reading.

Safety

  • Only use medicines prescribed for you, from a licensed pharmacy.
  • Follow your pharmacist’s instructions on diluent type, storage and how long the mixed vial stays usable.
  • Don’t change the diluent volume without recalculating: more water means more units for the same dose.
  • The FDA has warned that some peptide substances used in compounding may present significant safety risks.

Frequently asked questions

How many units is 250 mcg from a 5 mg vial with 2 mL water?

10 units (0.1 mL) on a U-100 syringe.

How much bacteriostatic water should I add?

Use the volume on your prescription or pharmacy label. The tool shows how the volume changes the units you draw.

About this tool

Written by
[Author: to be assigned]
Reviewed by
Expert review pending
Last updated
31 Oct 2026

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